Figure 2. Fractionation of F. tularensis reveals the presence of an inducible HMW carbohydrate in host-adapted bacteria.

Whole cells (WC) of MHB- (M) and BHI-(B) grown F. tularensis LVS were Tx114 phase-partitioned into aqueous (A), detergent (D), and Tx-insoluble phases. The insoluble material was treated with 0.2% sarkosyl to generate soluble (SS) and insoluble (SI) fractions. A) Intact fractions derived from 10 µg of WC protein were probed by western blot for the indicated proteins. Predicted locations were derived from the LVS protein sequence with the aid of PSORT (http://psort.hgc.jp/form.html). B) Carbohydrates present in proteinase K-treated fractions derived from 10 µg of WC protein were visualized by Emerald Green staining of resolved samples.