Figure 4. F. tularensis grown in BHI produce a HMW carbohydrate, independent of OAg, which correlates with reduced access of OMPs to Ab.

A) Carbohydrates from the indicated proteinase K-treated samples were visualized as in Fig 2b. B) Ab pulldown assays were performed as in Fig 3. C) surface-bound Ab to FopA ratios from 3 independent experiments were quantified as in Fig 3. Asterisks indicate values that differed significantly (p≤0.05, Bonferonni-corrected T-test).