Figure 7. BHI-grown F. tularensis SchuS4 also displays increased production of HMW carbohydrate(s) and reduced cytokine elaboration.

F. tularensis LVS (WT and wbtA) and SchuS4 were grown in MHB (M) and BHI (B). A) Proteinase K-treated lysates were resolved through 8-16 % SDS-PAGE Protean II large gels and stained with Emerald green to visualize carbohydrates. B) Viable F. tularensis SchuS4, grown as indicated, were co-incubated with C57BL/6 BMDMs at an MOI of 100 for 24 hrs prior to analysis of secreted cytokine levels. Results are means and standard errors from three independent experiments each conducted in duplicate.