Figure 2. Expressions of miR-296-3p, miR-296-5p, miR-127, miR-127-5p and miR-483 imprinted miRNAs in male germ-line (GS) and multipotent adult germ-line (maGS) stem cells.

Sperm and Embryonic stem (ES) cells were used as controls for comparison. All data were normalized to endogenous sno202 RNA as internal controls and calibrated on the STO cells, whose expression was considered one for all genes except for the miR-127 and miR-5p, for which sperm were used as a calibrator and considered one. Values above the bars indicate relative abundance of respective miRNAs. Different alphabet (a, b, c. d) on the bars indicate statistical difference (P<0.01) in gene expression.