Figure 3. Concentration-dependent inhibitory effect of various general and specific inhibitors on MDR probes accumulation in CHO K1 (panel A), HCT-15 (panel B), HepG2 (panel C) and HL-60/MX1 (panel D) cells.
The following MDR probes were used: eFluxx-ID® Green (filled squares), eFluxx® Gold (filled triangles), calcein AM (grey circles), DiOC2(3) (open triangles), CMFDA (open diamonds), pheophorbide A (filled diamonds). Cells were stained with the indicated probes in the presence of the various concentrations of the appropriate inhibitors. EC50 values are defined as the concentration of the inhibitor resulting in half-maximum inhibition of dye accumulation.