Table 1. Detection of the expression levels of three major types of MDR proteins in cultured cell lines using immunostaining with monoclonal antibodies and a flow cytometry assay.
Pgp Staining [C219] | MRP1 Staining [QCRL1] | BCRP/ABCG2 Staining[MM0047-2J39] | |
CHO K1 | 0.29±0.3 | 0.59±0.2 | 0.74±0.3 |
A549 | 0.12±0.02 | 0.63±0.3 | 0.57±0.2 |
HeLa | 0.06±0.01 | 0.10±0.03 | 0.14±0.01 |
HL-60 | 0.13±0.07 | 0.22±0.07 | 0.17±0.09 |
HL-60/MX1 | 0.11±0.05 | 0.31±0.04 | 0.83±0.01 |
HCT-15 | 0.28±0.2 | 0.60±0.13 | 0.07±0.02 |
HepG2 | 0.18±0.05 | 0.58±0.06 | 0.14±0.001 |
Average D values (± SEM) for three representative experiments are presented.