Table 3. Comparison of MDR activity detection in model cell lines overexpressing a single ABC transporter pump using MDR probes and inhibitors of different specificity.
| Cell Line | MDR Probe | Probe Specificity | CsA | Ver | MK571 | Nov |
| HCT-15 | Calcein AM | MDR1, MRP | 67.2±2.0 | 62.9±2.6 | 13.5±2.9 | 4.3±2.2 |
| eFluxx-ID® Green | 66.2±7.0 | 64.4±6.7 | 21.1±2.6 | 17.1±3.7 | ||
| eFluxx-ID® Gold | 68.5±6.6 | 65.8±6.0 | 16.2±2.6 | 16.6±1.7 | ||
| DiOC2(3) | MDR1 | 71.0±5.0 | 69.3±4.3 | 4.3±2.2 | 0 | |
| HepG2 | Calcein AM | MDR1, MRP | 59.0±2.4 | 22.0±2.9 | 40.7±4.5 | 3.0±0.1 |
| eFluxx-ID® Green | 62.9±4.0 | 20.3±1.6 | 58.8±3.2 | 17.3±1.2 | ||
| eFluxx-ID® Gold | 63.3±0.8 | 17.7±3.2 | 69.3±2.3 | 15.8±0.6 | ||
| CMFDA | MRP | 50.4±1.3 | 12.8±1.3 | 52.2±3.8 | 7.0±1.7 | |
| HCT-8 | Calcein AM | MDR1, MRP | 46.5±5.1 | 25.9±0.9 | 31.1±4.7 | 7.1±0.4 |
| eFluxx-ID® Green | 36.7±1.5 | 32.6±1.5 | 51.5±5.0 | 12.1±5.2 | ||
| eFluxx-ID® Gold | 33.8±2.7 | 29.0±0.9 | 48.4±5.6 | 3.5±1.9 | ||
| DiOC2(3) | MDR1 | 24.7±0.9 | 19.1±3.7 | 9.8±1.2 | 0 | |
| CMFDA | MRP | 43.0±3.2 | 21.2±3.2 | 39.2±3.6 | 0 | |
| HL-60 | Calcein AM | MDR1, MRP | 15.2±2.5 | 2.5±1.1 | 8.0±1.7 | 8.6±1.5 |
| eFluxx-ID® Green | 11.8±2.8 | 5.2±0.6 | 19.9±2.9 | 8.5±3.1 | ||
| eFluxx-ID® Gold | 0.29±0.3 | 14.0±3.4 | 15.8±0.6 | 7.2±1.6 | ||
| Pheophorbide A | BCRP | 6.5±1.7 | 6.8±0.6 | 7.6±1.6 | 8.5±1.4 | |
| HL-60/MX1 | Calcein AM | MDR1, MRP | 18.2±2.7 | 1.6±0.3 | 10.8±1.8 | 5.9±1.2 |
| eFluxx-ID® Green | 33.1±3.4 | 17.8±8.3 | 19.0±1.9 | 37.5±2.6 | ||
| eFluxx-ID® Gold | 30.2±3.4 | 14.6±2.9 | 16.1±3.8 | 24.6±3.9 | ||
| Pheophorbide A | BCRP | 22.5±3.0 | 7.6±3.8 | 10.2±3.7 | 23.4±0.8 |
Average MAF values + SEM for three representative experiments are provided. Negative MAF values have been replaced with 0.