Table 4. Examples of multiplex (dual) probe treatment for MDR activity profiling in the CHO K1 cell line.
Probes | Detection Channel | D-values Post Inhibitor Treatment(compared to uninhibited cells | |||
CsA | Verapamil | MK-571 | FTC | ||
eFluxx-ID® Green | FITC | 0.94 | 0.9 | 0.98 | 0.56 |
Pheophorbide A | PerCP | 0.25 | 0.14 | 0.15 | 0.33 |
eFluxx-ID® Gold | PE | 0.89 | 0.94 | 0.96 | 0.51 |
Pheophorbide A | PerCP | 0.25 | 0.11 | 0.14 | 0.34 |
eFluxx-ID® Gold | PE | 0.84 | 0.82 | 0.96 | 0.44 |
DiOC2(3) | FITC | 0.94 | 0.96 | 0.13 | 0.16 |
eFluxx-ID® Gold | PE | 0.9 | 0.94 | 0.85 | 0.53 |
CMFDA | FITC | 0.49 | 0.1 | 0.91 | 0.06 |
(Average D values for three representative experiments are provided as characteristics of MDR, with SD not exceeding 10% for each value).