Table 5. Examples of multiplex (triple) probe treatment for MDR activity profiling in the CHO K1 cell line.
| Probes | Detection Channel | D-values Post Inhibitor Treatment(compared to uninhibited cells | |||
| CsA | Verapamil | MK-571 | FTC | ||
| DiOC2(3) | FITC | 0.98 | 0.98 | 0.13 | 0.11 |
| eFluxx-ID® Gold | PE | 0.81 | 0.91 | 0.97 | 0.54 |
| Pheophorbide A | PerCP | 0.4 | 0.17 | 0.13 | 0.41 |
| CMFDA | FITC | 0.47 | 0.10 | 0.89 | 0.14 |
| eFluxx-ID® Gold | PE | 0.93 | 0.97 | 0.95 | 0.64 |
| Pheophorbide A | PerCP | 0.20 | 0.18 | 0.09 | 0.27 |
(Average D values for three representative experiments are provided as characteristics of MDR, with SD not exceeding 10% for each value).