Figure 1. Western blotting analysis showing the protein expression of CysLT1 and P2Y6 receptors in 16HBE14o- cells.
The expression of CysLT1 (44 kDa) and P2Y6 (41 kDa) receptors was demonstrated (lane 1), and their positions on the blot closely matched their calculated molecular masses of 39 kDa and 36 kDa, respectively. Detection of these protein bands appeared specific, as they were mostly blocked by prior reabsorption of the antibodies with their respective control antigen for 2 h at 4°C (lane 2).