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. Author manuscript; available in PMC: 2011 Jul 22.
Published in final edited form as: Biotechnol Bioeng. 2009 Dec 15;104(6):1047–1058. doi: 10.1002/bit.22517

Figure 3. Autoradiography and scintillation counting analysis of temperature-dependent effects on soluble protein production.

Figure 3

4-μL samples of total (T) and soluble (S) CFPS final reaction mixture product are separated by SDS-PAGE. Lowering R9-Nanog production temperature from 37° C to 25° C resulted in a slight improvement in soluble protein yield. This insight was also applied to R9-Oct3/4 and resulted in a significant improvement in soluble protein yield. Liquid scintillation data was averaged from n=3 experiments while the autoradiogram was selected from a representative experiment among the three.