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. Author manuscript; available in PMC: 2011 Jul 22.
Published in final edited form as: Biotechnol Bioeng. 2009 Dec 15;104(6):1047–1058. doi: 10.1002/bit.22517

Figure 4. Autoradiography for Yamanaka and Thomson reprogramming factor R9 fusion proteins produced using CFPS.

Figure 4

4-μL samples of total (T) and soluble (S) CFPS reaction products were separated by SDS-PAGE. All transcription factors are nona-arginine fusion proteins as described in Figure 1. The top row shows initial results obtained with KC6 extract and 37°C production temperature. The same panel of reprogramming factors was produced with BL21(DE3)Star cell extract at 25°C to generate improve yields of soluble and full-length proteins as shown in the bottom row. Representative images of individual lanes were selected from n=3 experiments.