Table 1. Experimental plan.

# Rats1	Procedure	Survival	Histological/Immunohistological procedures2
4	1 h I/R	12 h	AP histochemistry/IF LC3, LAMP1,CASPASE-3
9	1 h I/R	24 h	AP histochemistry/IF LC3, LAMP1,CASPASE-3/TUNEL assay
4	1 h I/R	48 h	AP histochemistry/IF LC3, LAMP1,CASPASE-3
2	1 h I/R	24 h	Intravitreal HRP 3 h before sacrifice, HRP histochemistry
2	1 h I/R	24 h	Intravitreal FITC-dextran 3 h before sacrifice
3	1 h I/R+3MA	24 h	IF LC3, LAMP1, CASPASE-3/TUNEL assay
2	1 h I/R	24 h	Immunoblot analysis LC3 I and II

¹For each animal, the left eye was injected, the right eye served as control.

²IHC was performed on 2 animals of each group.