Table 1. Primers used for cloning in the present study.
Primer | Position of 5′base | Sequence | Reference |
CTLA-4-1 | 1 | 5′-gaa gct tat ggc ttg tct tgg act c-3′ | AF130428 |
CTLA-4-2 | 483 | 5′-gga tat cat cag aat ctg ggc atg g-3′ | AF130428 |
CD27-1 | 178 | 5′-cta ggt acc atg gca tgg cc acct ccc-3′ | L24495 |
CD27-2 | 714 | 5′-cta gat atc gtc cga gct gca cag ggg-3′ | L24495 |
CD40L-1 | 13 | 5′-cta ggt acc atg ata gaa aca tac agc-3′ | X65453 |
CD40L-2 | 792 | 5′-cta gat atc gag ttt gag taa gcc aaa-3′ | X65453 |
HBcP-1 | 1284 | 5′-gat atc atg gac atc gac cct tat-3′ | V01460 |
HBcP-2 | 736 | 5′-ctc gag aca ttg aga ttc ccg aga-3′ | V01460 |
HBeP-1 | 1371 | 5′-gat atc atg caa ctt ttt cac ctc t-3′ | V01460 |
Reference: the Genbank accession numbers of the used reference sequences are given.
The 5′ extensions were added to the primers for cloning and are indicted as italic letters.