Figure 5. The nuclear localization of Zap1 is not affected by zinc.

Protease-deficient BJ2168 cells transformed with pZap1^WT were grown to exponential phase in LZM supplemented with 3 µM or 1000 µM ZnCl₂. BJ2168 cells lacking pZap1^WT were also grown in LZM supplemented with 1000 µM ZnCl₂. Total cell homogenates were separated into cytosolic and nuclear fractions as described in Materials and Methods. Equal amounts of protein from each sample (10 µg protein/lane) were assayed by immunoblotting using antibodies against Zap1 (myc), Pgk1, Dpm1, and Pho2.