Table 1.
Bacterial strains and plasmids
| Strains and plasmids | Relevant genotype | Reference or source |
|---|---|---|
| L. monocytogenes | ||
| EGD-e ΔtrpS × pFlo-trpS |
wild-type | T. Chakraborty (University of Giessen, Germany [36] |
| ΔtrpS,inlA/B × pFlo-trpS | [32] | |
| Lm-spa- | ΔtrpS,aroA,inlA/B × pFlo-trpS | This work |
| Lm-spa+ | ΔtrpS,aroA,inlA/B,int::Phly-spa × pFlo-trpS | This work |
| ΔtrpS × pSP0-PactA-gfp | [36] | |
| Lm-spa- × pSP0-PactA-gfp | ΔtrpS,aroA,inlA/B × pSP0-PactA-gfp | This work |
| Lm-spa+,aroA+ × pSP0-PactA-gfp | ΔtrpS,inlA/B,int::Phly-spa × pSP0-PactA-gfp | This work |
| Lm-spa+ × pSP0-PactA-gfp | ΔtrpS,aroA,inlA/B,int::Phly-spa × pSP0-PactA-gfp | This work |
| Plasmids | ||
| pFlo-trpS | TcR, | [36] |
| pSP0-PactA-gfp | EmR, gfp-ORF, actA-promoter | [36] |
| pLSV101intAB | EmR, ORIts, mutagenesis plasmid | [31] |
| pLSV101intAB::Phly-spa | spa-ORF, hly-promoter | This work |