Figure 4.

Per1-luc bioluminescence rhythms in liver explants treated with thapsigargin. (A) Representative daily records of Per1-luc expression in liver explants from AL (superior panel) and FR (inferior panel) rats during thapsigargin treatment (100 μM). The grey trace represents the DMSO control and the black trace the thapsigargin-treated culture. The vertical dotted line represents the former meal time of the FR rats. The horizontal black line above the upper panel indicates the onset and duration of drug treatment which ended with washout of the drug on day 6, marked by the horizontal gray dashed line. The subjective night (or dark phase) is marked only on the first day of culture. (B) Period from Per1-luc expression in liver cultures from AL (left bars) and FR (right bars) rats during thapsigargin treatment and after washout. All data plotted are presented as means ± SEM, n = 6. *Significant difference vs. the DMSO control, and ^x means difference against washout (Bonferroni post hoc test, α = 0.01).