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Acquisition of meiotic and developmental competence following culture in a two-dimensional (2D) system. Oocytes from a 2D culture system (white bars; n = 218) have significantly greater incidence of fertilization than oocytes from the three-dimensional alginate system (black bars; n = 163), as documented by the presence of two PN (2PN) after IVF* (P < 0.001). No significant difference was found in the percentage of oocytes able to reach the MII stage (P > 0.05).
