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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: J Nat Prod. 2011 Jun 10;74(7):1621–1629. doi: 10.1021/np200336g

Figure 3.

Figure 3

Panel A shows minimum inhibitory concentration (MIC) of starting material (sm), berberine alone, and berberine in combination with 11 fractions (each at a fixed concentration of 75 μg/mL) from the first stage of separation (flash chromatography over silica gel with CHCl3:MeOH gradient) of the Hydrastis candensis leaf extract. The MIC of berberine (75 μg/mL) was reduced significantly by combining with the starting material and with several of the fractions, with the most active fraction being fraction 4. Panels B and C show the distribution of flavonoids (measured with negative-ion LC-MS), and alkaloids (measured with positive-ion LC-MS), respectively in the fractions. Fraction 4 contains the highest levels of the 3 flavonoids. There is a moderate multiple correlation between MIC and flavonoid peak area (R = 0.77, p = 0.016).