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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Placenta. 2011 Jun 12;32(8):603–610. doi: 10.1016/j.placenta.2011.05.008

Figure 4.

Figure 4

PSG17N binds to Sog9 cells expressing CD9 (Sog9-CD9) while PSG23 does not. (A)Sog9 cells were stably transfected with a plasmid encoding murine CD9. Expression of CD9 in the sorted cells was verified with anti-CD9 mAb. Sog9-CD9 cells were incubated with PSG17N-Fc (B) or PSG23N-Fc (C). Protein binding was detected with PE-conjugated anti-human Fcγ. (D) BHK-21 cells transfected with mCD9 or empty plasmid were added to Petri dishes coated with PSG17N, PSG23N or FLAG-Fc. After several washes, the cells bound to the dish were counted in three separate fields.