Fig. 7. Replication and translation of TMV RNA encoding MP or sb-9 in protoplasts. (A and B) Northern analyses of positive-stranded and negative-stranded viral RNA, respectively. (C and D) Western blot analyses of CP and MP accumulation, respectively. Bands corresponding to TMV RNA and MP and CP sgRNAs are indicated. Lanes 1 and 2, N.tabacum protoplasts infected with TMV RNA encoding MP or sb-9, respectively; lanes 3 and 4, N.benthamiana protoplasts infected with TMV RNA encoding MP or sb-9, respectively. The numbers on the left indicate molecular mass standards in thousands of daltons.