Fig. 8. Effect of mimicking MP phosphorylation on viral cell-to-cell and systemic movement. Ten days after infection, leaves were harvested from plants inoculated with TMV RNA encoding wild-type MP or sb-9 and analyzed for the presence of viral CP as described in Materials and methods. (A) Nicotiana tabacum plants. Lane 1, TMV CP; lane 2, uninfected plant; lanes 3 and 4, inoculated and uninoculated leaves, respectively, of wild-type plants infected with TMV RNA encoding wild-type MP; lanes 5 and 6, inoculated and uninoculated leaves, respectively, of wild-type plants infected with TMV RNA encoding sb-9; lanes 7 and 8, inoculated and uninoculated leaves, respectively, of MP transgenic plants infected with TMV RNA encoding sb-9. (B) Nicotiana benthamiana plants. Lane 1, TMV CP; lane 2, uninfected plant; lanes 3 and 4, inoculated and uninoculated leaves, respectively, of plants infected with TMV RNA encoding sb-9; lanes 5 and 6, inoculated and uninoculated leaves, respectively, of plants infected with TMV RNA encoding wild-type MP. The arrowhead indicates the position of TMV CP. Protein molecular mass standards are indicated on the left in thousands of daltons.