Figure 5. IL-1β increases intracellular and secreted levels of NGAL in SEB-1 sebocytes.
SEB-1 sebocytes were treated with 50ng/ml of IL-1β for 24 h. a. Both the cell lysate and culture medium were assayed for NGAL by ELISA. b. The culture medium was assayed for IL-8 by ELISA. All values are expressed as a fold change compared to vehicle. Data was analyzed using a Student’s t-test, n=3. *, p<0.05.