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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Reprod Toxicol. 2011 May 20;32(1):112–123. doi: 10.1016/j.reprotox.2011.05.007

Fig. 6. Effect of CrVI on cyclins and PCNA in immortalized granulosa cells, SIGC.

Fig. 6

SIGC were pre-treated with or without vitamin C (1 mM) for 24 h and treated with CrVI (10 μM) for 0 h, 12 h and 24 h period. Western blots were performed with 75 μg of protein, as described in Materials and Methods section. A, Representative western blots of cyclins D1, D2, D3, E2, A, B1, PCNA and b-actin. B, Histograms of Integrated Density Value (IDV) for each protein, normalized toβ-actin. Each value is the mean ± SEM from 3 different plates per treatment, P<0.05; a: CrVI-treatment, 0 h vs 12 h or 24 h; b: Vitamin C vs CrVI+Vitamin C-treatment, 0 h vs 12 h or 24 h; c: CrVI (12 h or 24 h) vs CrVI+Vitamin C (12 h or 24 h).