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On the day of an experiment, ewes were placed in a modified metabolism cart so that the animal’s head was inside a plexiglass chamber. A vinyl diaphragm attached to the open side of the chamber was drawn around the animal’s neck to isolate the atmosphere in the chamber from ambient air. Subjects were exposed to increased inspired fractional concentrations of carbon dioxide for 60 minutes. The percentage of oxygen and carbon dioxide in the chamber was measured using a gas analyzer.
