Figure 2.

Sequence, secondary structure and activity of a ykkC RNA representative. (A) Bacillus subtilis ykkC RNA construct used for reporter assays with integration vector pDG1661. The reporter plasmid contains a ribosome binding site and start codon that follow ∼50 bp after the inserted riboswitch candidate.²³ Mutants M1 and M3 were designed to disrupt base-pairing in P1 and M2 disrupts a highly-conserved region of the RNA. M4 deletes the right shoulder of the putative expression platform terminator stem. (B) β-galactosidase expression levels measured in Miller units using methods described previously in reference ¹⁴, for wild-type and the various mutants described in (A) under minimal and rich media (M4 only) as indicated. When present, error bars represent standard deviation among three independent replicates.