Figure 3.

Knock down of c-Myc protein reduces proliferation of C4-2 cells. C4-2 cells were first transfected with either a non-specific control SMART-pool or c-Myc siRNA. Two days later, cells were detached using trypsin-EDTA and total cell number in each well was measured using a Z1 Coulter Counter. Each bar represents the mean ± SD for three wells. *p < 0.05 when compared to DMSO control. A representative experiment is shown. Protein gel blot analysis was used to determine the levels of c-Myc and actin protein in transfected cells.