Figure 8.

Troglitazone decreases c-Myc protein independently of PPARγ. (A) C4-2 cells were treated with ethanol (EtOH) or DMSO vehicle, ciglitazone (Cig, 45 εM), pioglitazone (Pio, 45 εM), troglitazone (Trog, 45 εM) or rosiglitazone (Ros, 45 εM) for 48 h. The amount of c-Myc and actin protein in treated cells was then determined using protein gel blot analysis. (B) C4-2 cells were first transfected with either a non-specific control SMARTpool or PPARγ SMARTpool siRNA. Two days later, the cells were treated with DMSO vehicle or troglitazone 45 εM for 48 h. Protein gel blot analysis was used to determine levels of c-Myc, PPARγ and actin protein in treated cells. (C) C4-2 cells were treated with DMSO vehicle or troglitazone 45 εM in the presence or absence of the PPARγ antagonist GW9662. Following treatment, the levels of c-Myc and actin protein in treated cells was measured using protein gel blot.