Figure 1.

Immunoblot analysis of Hsp87 and Hsp85 (A), and Hsp75 and Hsp73 (B) in sorghum. The 24-h-old seedlings were treated for 24 h with LaCl₃ and verapamil at 37°C and exposed to heat shock at 45°C for 3 h. 25 µg of total proteins were resolved on 8% SDS-PAGE, transferred on to Hybond C membrane and probed with anti-N. crassa Hsp80 and anti-S. bicolor Hsp70 polyclonal antibodies. Densitometer scanning shows the fold change in intensity of Hsp85 (□) and Hsp73 (□) compared to the pre-treatment stage (48 h), whereas the intensity of Hsp87(▪) and Hsp75 (▪) is relative to the water grown heat-stressed seedlings (45°C) in this and subsequent frames. The data were subjected to one way analysis of variance. (A): Significant difference at p ≤ 0.05 with respect to 37°C water-grown control at 51 h. (B): Significant difference at p ≤ 0.05 with respect to 45°C water-grown seedlings.