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. 2011 Mar 1;6(3):400–405. doi: 10.4161/psb.6.3.14337

Figure 4.

Figure 4

Label-free binding of Flag-BRI1 cytoplasmic domain to immobilized GST-BAK1 cytoplasmic domain requires millimolar concentrations of free Mg2+. Binding was measured using the ForteBio Octet, which uses fiberoptic sensors to detect protein:protein interactions via biolayer interferometry.