Skip to main content
. Author manuscript; available in PMC: 2012 Jan 27.
Published in final edited form as: Transplantation. 2011 Jan 27;91(2):176–182. doi: 10.1097/TP.0b013e3181fffff2

Fig. 4. Role of HO1 in FD-induced protection of human renal tubular cells against cold hypoxic injury: studies using HO1 siRNA.

Fig. 4

Fig. 4

a: Real-time PCR data: the inhibitory effect of HO1 siRNA on hemin- and FD-induced HO1 mRNA is displayed (average fold change over control based on result from 3 experiments in triplicate is shown).

b: HO1 siRNA reverses FD’s cytoprotection against cold hypoxic cell injury. Control cells, preconditioned with saline instead of FD, sustained marked injury from 48-h of cold hypoxic storage. The transfecting agent oligofectamine (OF) or HO1 siRNA alone had no significant effect on cold storage injury. FD preconditioning was associated with significant protection against cold storage injury, which was significantly reversed by HO1 siRNA and OF treatment (n=4 in duplicates; *P<0.05 vs. the rest and **P<0.05 vs. the rest, by ANOVA).