Ulk2 is expressed in the Hb and colocalizes with Kctd12.1. A–C, ulk2 transcript is detected by in situ hybridization in many neurons of the CNS, but is enriched in the paired Hb (white outlines) at 48, 72, and 96 h post fertilization. D–F, A combination of fluorescent in situ for ulk2 transcript and Kctd12.1 immunofluorescence reveals coexpression in the left lateral Hb. G, H, Transgenic expression of GFP:Ulk2 fusion protein in a single neuron of the left lateral Hb shows localization of GFP:Ulk2 to puncta in Hb dendrites (arrows). I–I″, GFP:Ulk2 colocalizes with Kctd12.1. The image in I is an optical section through the left Hb in G and H with medial to the right and dorsal to the top; dashed lines in I indicate the relative orientation of optical sections shown in I′ and I″. Scale bars: A, D, 50 μm; G–I, 15 μm.