Table II.
Test Protein Results.
| Protein | Test Round | Crystals | # w/no Cond 4* |
Notes |
|---|---|---|---|---|
| T.t. 46 SS DNA-spec. Exonuclease (m + r) | 1 | 3 | 3 | |
| T.t. 71 Intracellular protease/amidase (m + r) | 1 | 22 | 5 | |
| T.t. 75 Prolyl endopeptidase (m) | 1 | 4 | 1 | |
| T.t. 97 Aspartate Racemase (m + r) | 1 | 11 | 5 | |
| T.t. 101 Amino Peptidase ( r ) | 1 | 1 | ||
| T.t. 103 Acid Phosphatase (m) | 1 | 6 | 2 | 1 |
| 2 | 5 | 2 | 2 | |
| T.t. 105 2-hydroxyacid Dehydrogenase (m) | 1 | 9 | 4 | |
| T.t. 169 RNAse (m) | 1 | 5 | 0 | |
| T.t. 185 Nucleotide Kinase (m) | 1 | 4 | 2 | |
| T.t. 187 Mg-dependent DNAse (m) | 1 | 5 | 3 | |
| T.t. 189 Nucleoside Diphosphate Kinase (m) | 1 | 16 | 5 | |
| P.v. 01 unknown protein (m) | 1 | 6 | 3 | |
| ProtX - provided by J. Gavira, Granada ( r ) | 1 | 7 | 3 | 3 |
*
Optimizations where crystals were not obtained in the 4th (stock conditions) capillaries.
1
3 unique to round 1, temp. reduced to 13°C
2
2 unique to round 2
3
only 12 leads tested
m = manual & r = robotic plate setup.