Table 2.
Community composition based on CARD-FISH analysis
| Samples | % of cell count 1 | % of aggregate count 1 | % of biovolume1 |
|---|---|---|---|
| S1 | |||
| ANME-1 | Below detection limit2 | Below detection limit2 | Below detection limit2 |
| ANME-2 | 8.2 ± 3.0 | 37.1 ± 6.2 | 13.4 ± 4.2 |
| ANME-3 | 0.1 ± 0.1 | 2.1 ± 1.4 | 1.5 ± 1.5 |
| SRB | 2.9 ± 1.5 | 32.0 ± 6.2 | 22.7 ± 5.3 |
| S2 | |||
| ANME-1 | Below detection limit3 | Below detection limit3 | Below detection limit3 |
| ANME-2 | 2.5 ± 2.0 | 47.2 ± 8.2 | 50.4 ± 15.9 |
| ANME-3 | 0.1 ± 0.1 | 0.8 ± 0.7 | 2.4 ± 1.8 |
| SRB | 0.8 ± 0.4 | 37.6 ± 5.0 | 60.6 ± 5.5 |
1 The average value and standard error were calculated based on 50 fields of view on each hybridization. No ANME-1 cell or aggregate was observed based on our method. 2 Detection limit of 4 × 104 cells/ml slurry. 3 Detection limit of 9 × 104 cells/ml slurry