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. Author manuscript; available in PMC: 2011 Jul 24.
Published in final edited form as: Methods Enzymol. 2011;487:431–463. doi: 10.1016/B978-0-12-381270-4.00015-9

Figure 15.7.

Figure 15.7

The power of the MCCP algorithm to detect short-duration (transient) states in the myosin V ATPase cycle, specifically, the short-lived detached state after the motor head releases from actin but before it steps and rebinds, is determined from photon emission rates simulated using the angles in Table 15.2. Simulations with np = 8 (left) and np = 16 polarization channels (right) indicate one (top), two (middle), or three (bottom) detected changepoints as the number of photons in the transient state is increased from 0 to 1200 for various SBRs. Requiring that the interval be detected with at least 90% accuracy (dashed curves, middle panels) significantly increases the number of photons needed to identify the state reliably (see text).