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. Author manuscript; available in PMC: 2011 Jul 24.
Published in final edited form as: Methods Enzymol. 2011;487:431–463. doi: 10.1016/B978-0-12-381270-4.00015-9

Table 15.2.

Orientation and wobble (δ) used in the simulations of myosin V stepping (see Section 2.1)

State {θ, φ} {β, α} δ
Prestep {96.7, 168.8} {20, −20} 40
Detached head 90
Poststep {18.9, 23.3} {80, −85} 40

The orientations are represented in polar coordinates, in the microscope (θ, φ) and actin (β, α) frames. That is, β is the polar angle of the probe with respect to the actin filament and α is the azimuthal angle around the filaments, where α = 0 is parallel to the microscope stage and α = 90 is parallel to the optical axis of the microscope. All angles are in degrees.