Supplementary Fig. 1.

Effect of alkylating agents on the growth of Trypanosoma cruzi strains in the presence or absence of cadmium. Exponential cultures of epimastigotes from different T. cruzi strains were grown in LIT media containing (A) 10, 20 and 30 μM cisplatin; (B) 30 μM cisplatin in the presence or absence of 3 μM Cd²⁺; (C) 25, 50 and 100 μM MNNG or (D) 100 μM MNNG in the presence or absence of 3 μM Cd²⁺. Viable cell numbers were determined after 3 days (for MNNG treatment) or 5 days (for cisplatin treatment) with erythrosine (B). Results are shown as mean ± SD of 3 independent experiments performed in triplicate.