Supplementary Fig. 4.

TcMSH2 levels among different T. cruzi strains. In A: Northern blot analysis. Twenty-five micrograms of total RNA extracted from epimastigotes of Col1.7G2, Silvio X-10 cl1, JG and Esmeraldo cl3 were hybridized with the same radiolabeled probe indicated in Supplementary Fig. 6E. The bottom part of the figure shows ethidium bromide staining of rRNA bands to indicate the amount of RNA loaded on the gel. In B: Western blot analysis. Protein extracts were separated by SDS-PAGE and then transferred to nitrocellulose filters, and TcMSH2 were detected using polyclonal antibodies anti recombinant TcMSH2.