Fig. 4.

Survival of ΔTcmsh2::HYG/TcMSH2 clones in the presence of cisplatin and H₂O₂ and accumulation of 8-oxoguanine after oxidative stress damage. Wild type and ΔTcmsh2::HYG/TcMSH2 clones were treated in early log phase with 25 and 50 μM cisplatin (A) or 200 and 300 μM H₂O₂ (B). After 5 days, viable cell numbers were determined using erythrosine B dye exclusion. Accumulation of 8-oxoG in the nucleus and kinetoplast DNA in wild type and ΔTcmsh2::HYG/TcMSH2 mutants was measured before and after treatment with 300 μM H₂O₂ (C). Each bar represents the mean ± SD of 100 cells analyzed. Statistical differences (p ≤ 0.05) were observed between clone 2 and wt cells in the analysis of kDNA both in untreated and treated parasites.