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. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: Mol Plant Microbe Interact. 2010 Jan;23(1):49–57. doi: 10.1094/MPMI-23-1-0049

Fig. 1.

Fig. 1

AvrM proteins interact with the M protein in yeast. A and B, Growth of yeast strain AH109 expressing GAL4-activation domain (AD) and -binding domain (BD) fusion proteins on minimal media lacking histidine. Growth indicates expression of the HIS3 reporter gene as a result of interaction between the GAL4-AD and GAL4-BD fusion proteins. A, AD and BD proteins were expressed alone (–) or as fusions to AvrM-A (A), avrM (a), or M. B, Co-expression of the AD–M fusion protein and the BD–AvrM (A to E) or BD–avrM fusion proteins. C, Protein extracts from yeast strain AH109 expressing AD–M, AD–AvrM (A to E), or BD–avrM were analyzed by immunoblotting with anti-GAL4-AD (α-AD) or anti-cMyc (α-cMyc). Positions and sizes of protein molecular mass standards are indicated.