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. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: J Thromb Haemost. 2009 Oct 23;8(1):185–193. doi: 10.1111/j.1538-7836.2009.03662.x

Figure 3. MMP-2, but not MMP-9, is activated during VEGF-stimulated EPC differentiation.

Figure 3

(A) Zymography assay. After EPCs embedded in collagen gel were cultured with or without 25 ng/mL of VEGF for 24 hours, the gelatinolytic activity in the conditioned culture media was analyzed by zymography. (B) VEGF (25 ng/ml)-stimulated expression and secretion of MMP-2 is time-dependent. The expression of cytosolic MMP-2 in EPCs as determined by Western-blot with β-tubulin serving as a loading control (i); Zymography of the conditioned culture media (ii). The results are representative of three independent experiments.