FIG. 7.

(A–C) H&E, Masson's trichrome, and von Kossa staining of PLGA constructs indicated calcification distributed sparsely in the constructs (dark spots, indicated by arrows). 200×, scale bars=100 μm. (D–F) In contrast, staining for PGS, P-PGS, and Pl-P-PGS showed no calcium deposition. 200×, scale bars=100 μm. (G) Quantification of ALP activity in all groups indicated that cells in PLGA scaffold have significantly higher ALP activity than those in all PGS groups, n=4, **p<0.01. PLGA, poly(lactic-co-glycolic acid); ALP, alkaline phosphate. Color images available online at www.liebertonline.com/tea