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. 2011 Aug;165(2):163–171. doi: 10.1111/j.1365-2249.2011.04424.x

Fig. 4.

Fig. 4

Phosphorylation of signal transducer and activator of transcription (STAT)-3 and STAT-1 in peripheral blood mononuclear cells (PBMCs) at different times following recombinant human interleukin (rhIL)-10 stimulation. PBMCs were stimulated with rhIL-10 (10 ng/ml) or left unstimulated (0 min), and collected at 5 min, 15 min and 30 min; phosphorylation of STAT-3 and STAT-1 was detected by flow cytometry. (a) Representative flow cytometric histograms for phosphorylation of STAT-3 and STAT-1 from an active systemic lupus erythematosus (SLE) patient and a healthy control. Shaded histograms represent unstimulated PBMCs (0 min). (b) Analysis of time–courses for phosphorylation of STAT-3 and STAT-1 in controls and SLE patients. (c) Analysis of time–courses for phosphorylation of STAT-3 and STAT-1 in controls, active patients, and inactive patients. (d) Analysis of time courses for phosphorylation of STAT-3 in controls, LN, and non-LN group (left), and in different subgroups (right); y-axes represent average mean fluorescence intensity (MFI) and x-axes represent time–courses after rhIL-10 stimulation. pSTAT3: phosphorylation of STAT-3. pSTAT1: phosphorylation of STAT-1. A LN: active LN patients; A non-LN: active non-LN patients; In LN: inactive LN patients; In non-LN: inactive non-LN patients. *P < 0·05; ***P < 0·001.