Fig. 1.
Role of FcγRIIB on glucuronoxylomamman (GXM)-induced Fas ligand (FasL) surface up-regulation. MonoMac6 cells (1 × 106/ml), pretreated and non-treated with antibody to FcγRIIB (0·1 µg/ml) were cultured for 2 h in the presence or absence of GXM (100 µg/ml) and analysed by flow cytometry or Western blotting. (a) Percentage of FasL-positive cells. Bars represent the mean ± standard error of the mean (s.e.m.) of three experiments. (b) Western blotting for FasL. Actin was used as loading control. Optical density (OD) of reactive bands was measured and normalized by the actin intensity in the same lane. FasL level was quantified in relation to untreated cells. Blots are representative of results obtained from five separate experiments with similar results. The fold increase is the mean ± s.e.m. of five experiments. (a,b) Incubation with irrelevant goat polyclonal immunoglobulin (Ig)G did not affect FasL expression. *P < 0·05 (antobody to FcγRIIB plus GXM-treated, versus GXM-treated).