Figure 1.
Design of pS100β-YC 3.60 from previously derived vectors. A, S100β-EGFP(a1), pCMV-YC 3.60(a2), and the S100β-YC 3.60(a3) construct. Restriction enzyme sites are labeled. The NotI site 5' to YC 3.60 in a2 was mutated by PCR to an AgeI site for compatibility with the 3' end of the S100β promoter.