Figure 1.
Calorie restriction decreases intracellular NADH levels. Measurements of intracellular levels of NAD (A,C) and NADH (B,D) in various yeast strains grown in 2% or 0.5% glucose. Results show average of three independent experiments, each conducted in duplicate. Error bars denote standard deviations. NAD and NADH levels are shown as levels of NAD or NADH (in micromolar) normalized to the concentrations of proteins from the extracts (in micrograms) present in the reaction. Reactions contain cell extracts from 105 cells. (WT) Wild-type yeast strain PSY316; (+Hap4-oe) wild-type cells overexpressing Hap4; (2%) cells grown in 2% glucose; (0.5%) cells grown in 0.5% glucose. npt1Δ, cyt1Δ, and sir2Δ hmr1Δ are isogenic derivatives of PSY316.