Table 1.
Intracellular concentrations of NAD and NADH
| Normal condition (mM)
|
Calorie restriction (mM)
|
|||||
|---|---|---|---|---|---|---|
| NAD | NADH | NAD/NADH ratio | NAD | NADH | NAD/NADH ratio | References |
| 2.14a,b | — | — | — | — | — | Smith et al. 2000 |
| 1.14a,c | — | — | — | — | — | Ashrafi et al. 2000 |
| 2-3 | — | — | 2-3 | — | — | Lin et al. 2001 |
| 2a,d | 0.78a,d | 2.56 | — | — | — | Anderson et al. 2002 |
| 1.26 ± 0.06e | 0.85 ± 0.13 | 1.48 | 1.19 ± 0.08 | 0.39 ± 0.11 | 3.05 | This study |
a
We converted reported units into millimolar (per cell) assuming a yeast cell size of 70 fL (70 μm3) and the total protein of 6 pg/cell (Sherman 2002).
b
Reported unit was 1.5 × 10-4 pmole/cell.
c
Reported unit was ∼80 amole/cell.
d
Reported units were 23.7 amole/pg protein and 9.3 amole/pg protein for NAD and NADH, respectively.
e
Numbers show mean ± standard deviations derived from data shown in Fig. 1.