Table 2.
Primers | Sequences (5′ → 3′) | Target | Annealing temperature (°C) | Amplicon length (bp) | References |
---|---|---|---|---|---|
27f | AGA GTT TGA TCM TGG CTC AG | Bacteria | 54 | 1,535 | [19] |
1525r | AAG GAG GTG WTC CAR CC | ||||
340f | TCC TAC GGG AGG CAG CAG T | Bacteria | 60 | 467 | [20] |
806r | GGA CTA CCA GGG TAT CTA ATC CTG TT | ||||
TaqMan probe | 6-FAM-5′-CGT ATT ACC GCG GCT | 70 | |||
GCT GGC AC-3′-TAMRA | |||||
Bac303f | GAA GGT CCC CCA CAT TG | Bacteroides and Prevotella | 56 | 418 | [21] |
Bac708r | CAA TCG GAG TTC TTC GTG | ||||
ARC787F | ATT AGA TAC CCS BGT AGT CC | Archaea | 60 | 273 | [22] |
ARC1059R | GCC ATG CAC CWC CTC T | ||||
Ra1281f | CCC TAA AAG CAG TCT TAG TTC G | R. albus | 55 | 175 | [23] |
Ra1439r | CCT CCT TGC GGT TAG AAC A | ||||
Fs-f | GGT ATG GGA TGA GCT TGC | F. succinogenes | 63 | 446 | [24] |
Fs-r | GCC TGC CCC TGA ACT ATC | ||||
530f | GTG CCA GCM GCC GCG G | Butyrivibrio | 65 | 371 | [19] and this study |
Buty-900r | TGC GGC ACY GAC TCC CTA TG | ||||
Sel-Mit-f | TGC TAA TAC CGA ATG TTG | S. ruminantium and M. multacida | 53 | 513 | [24] |
Sel-Mit-r | TCC TGC ACT CAA GAA AGA | ||||
Ram-f | CAA CCA GTC GCA TTC AGA | Ruminobacter amylophilus | 57 | 642 | [24] |
Ram-r | CAC TAC TCA TGG CAA CAT | ||||
Rf154f-K | TCT GGA AAC GGA TGG TA | R. flavefaciens | 55 | 295 | [23] |
Rf425r-K | CCT TTA AGA CAG GAG TTT ACA A |