. Author manuscript; available in PMC: 2012 Apr 22.

Published in final edited form as: Biochem Biophys Res Commun. 2011 Mar 23;407(4):703–707. doi: 10.1016/j.bbrc.2011.03.084

Table 3.

Effect of TPEN on T-bet and IFN-γ mRNAs in HUT-78 cells after Con-A Stimulation¹

	T-bet/GAPDH mRNA	IFN-γ/GAPDH mRNA
A: Con-A, 24h	0.088±0.021^*	0.0087±0.0032
B: TPEN 30 min; Con-A, 24h	0.015±0.016	0.0027±0.0031
C: Zinc plus pyrithione, 30 min; Con-A, 24h	0.095±0.016^*	0.0120±0.0066^*
D: Zinc (extracellular 20 uM zinc), 30 min	0.001±0.001	0.0003±0.0004
E: Pyrithione, 30 min	0.017±0.010	0.0034±0.0037

:HUT-78 cells were incubated with 5 μM TPEN, a zinc-specific chelator, or 20 μM zinc plus 10 μM pyrithione, a zinc ionophore for 30 min, and then following change of media, were continously incubated with Con-A for 24h. The cells were harvested for determination of T-bet and IFN-γ mRNAs by real time RT-PCR.

:p<0.05 (n=3) by ANOVA analysis (Dunnett post hoc Multiple comparison for Gr B vs Gr A, C, D, or E).