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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Mar 23;407(4):703–707. doi: 10.1016/j.bbrc.2011.03.084

Table 4.

Effect of zinc on relative mRNA levels for Th1 and Th2 type cytokines, T-bet in HUT-78 cells1

Relative Cytokine mRNA PMA/PHA stimulation p value IL-12 stimulation p value
Zn− Zn+ Zn− Zn+
IL-2 (×10−3) 1.2±1.2 5.8±9.6 0.06 0.85±0.79 2.5±4.9 0.162
IFN-γ (×10−6) 0.45±0.18 8.7±8.3 0.011 4.1±0.97 3.3±0.31 0.13
IL-4 (×10−7) 2.0±0.42 1.7±0.37 0.20 2.6±0.4 1.2±0.30 0.004
IL-12Rβ2 (×10−5) 0.1±0.03 0.59±0.14 0.019 0.65±0.08 3.58±0.67 0.004
T-bet (×10−5) 0.23±0.09 0.84±0.34 0.007 1.2±0.15 1.4±0.79 0.33
1

; zinc-treated HUT-78 cells were stimulated with PMA (5 ng/mL)/PHA (10 μg/mL) or IL-12 (100 pg/mL). Relative mRNA levels to 18S RNA were determined by real time RT-PCR (n=3).